General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter EDG2 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0839C3)
The PathHunter EDG2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of EDG2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged EDG2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting EDG2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Amsbio LLC MIP-2 Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
MIP-2 Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
Miltenyi Biotec, Inc. aIntegrin b7-PE-Vio615,h, m, REA441,100t
Identification and enumeration of integrin β7+ cells by flow cytometry.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Biochain Institute Inc Frozen Tissue Section - Parkinson's Disease: Brain: Temporal Lobe, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx PathHunter ADRA2C Total Internalization U2OS Cell Line, 2 vials. (93-0923C3)
The PathHunter ADRA2C Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of ADRA2C via detection of localization to the early endosome. The included cell line overexpresses EA-tagged ADRA2C and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADRA2C receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay LPA3 Lysophospholipid Receptor Frozen Cells, 2 Vials of Cells. (HTS130RTA)
The Ready-to-Assay LPA3 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. LPA) based activation of LPAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LPAR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of LPAR3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen BB1 Bombesine/Neuromedin B Receptor Stable Cell Line, 2 vials. (HTS123C)
The ChemiScreen BB1 Bombesin/Neuromedin B Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Lonza Walkersville P4 PC 384-well Nufe Kit L
The P4 Primary Cell 384-well Nucleofector Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT Nucleofector System This kit has been determined to be optimal for transfecting following cell types B cell (mouse) DC (mouse) immat MEF (mouse) The respective cell type-specific Optimized Protocols or recommendations are available under Instructions or in our Lonza Bioscience knowledge database In these Optimized Protocols the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of individual cell types The kit is available in different variants for either 2 x 384 or 10 x 384 reactions
Genscript Corporation CHO-K1/4-1BB Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human TNF receptor superfamily member 9 (4-1BB) on the surface The surface expression of 4-1BB is validated by FACS analysis
Eurofins Discoverx PathHunter HEK 293 EDG3 β-Arrestin Cell Line, 2 vials. (93-0217C1)
The PathHunter EDG3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of EDG3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG3 and EA-tagged β-Arrestin-2. Activation of EDG3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS EphA4 Functional Assay, 2 vials. (93-0877C3)
The PathHunter U2OS EphA4 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphA4 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphA4 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
